Normal FHIT transcripts in renal cell cancer- and lung cancer-derived cell lines, including a cell line with a homozygous deletion in the FRA3B region.

van den Berg, A; Draaijers, T G; Kok, K; Timmer, T; Van der Veen, A Y; Veldhuis, P M; de Leij, L; Gerhartz, C D; Naylor, S L; Smith, D I; Buys, C H

van den Berg, A; Draaijers, T G; Kok, K; Timmer, T; Van der Veen, A Y; Veldhuis, P M; de Leij, L; Gerhartz, C D; Naylor, S L; Smith, D I; Buys, C H.

Afiliación

van den Berg A; Department of Medical Genetics, University of Groningen, The Netherlands.

Genes Chromosomes Cancer ; 19(4): 220-7, 1997 Aug.

Article en En | MEDLINE | ID: mdl-9258656

RESUMEN

The recently identified FHIT gene encompasses the FRA3B region and the breakpoint of a constitutive t(3;8) occurring in a family with hereditary renal cell cancer. Occurrence of aberrant transcripts in different types of tumours has led to the suggestion that FHIT might play a critical role in the development of various types of cancer. We have analyzed the gene and its transcripts in lung cancers and renal cell cancer-derived cell lines. A lung adenocarcinoma cell line, GLC-A2, appeared to have a homozygous deletion in intron 5 of FHIT. RT-PCR analysis revealed a normal-sized PCR product in all of the cell lines: Including GLC-A2. A number of them had an additional aberrant product. Analysis of a great number of control cell lines and tissues showed that the majority of these also had aberrant PCR products in addition to a normal-sized PCR product. Different specimens of the same cell type showed variable additional RT-PCR products. Normal-sized PCR products had a sequence identical to the FHIT sequence. PCR products longer than normal had insertions of different sizes at different positions. With three exceptions, PCR products shorter than normal represented FHIT sequences missing one or more entire exons. Thus, the presence of aberrant transcripts is not cancer-specific. Conceivably, sequence responsible for the instability of the FRA3B region are being transcribed into FHIT pre-mRNA and may cause the abnormal splicing and processing of the transcripts.

Asunto(s)

Ácido Anhídrido Hidrolasas; Neoplasias Renales/genética; Neoplasias Pulmonares/genética; Proteínas de Neoplasias/genética; Proteínas/genética; Adenocarcinoma/genética; Carcinoma de Células Pequeñas/genética; Carcinoma de Células Escamosas/genética; Deleción Cromosómica; Mapeo Cromosómico; Cromosomas Artificiales de Levadura; Homocigoto; Humanos; Hibridación Fluorescente in Situ; Repeticiones de Microsatélite; Reacción en Cadena de la Polimerasa; ARN Neoplásico/aislamiento & purificación; Análisis de Secuencia de ARN; Transcripción Genética/genética; Células Tumorales Cultivadas

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas / Ácido Anhídrido Hidrolasas / Neoplasias Renales / Neoplasias Pulmonares / Proteínas de Neoplasias Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Genes Chromosomes Cancer Asunto de la revista: BIOLOGIA MOLECULAR / NEOPLASIAS Año: 1997 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Estados Unidos

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