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Estrogen does not induce the calcium-dependent nitric oxide synthase in cultured human uterine endothelial and myometrial smooth muscle cells.
Tschugguel, W; Zhegu, Z; Schneeberger, C; Tantscher, E; Czerwenka, K; Fabry, A; Wojta, J; Zeillinger, R; Huber, J C.
Afiliación
  • Tschugguel W; Department of Obstetrics and Gynecology, University of Vienna School of Medicine, Austria. walter.tschugguel@akh-wien.ac.at
J Vasc Res ; 34(4): 281-8, 1997.
Article en En | MEDLINE | ID: mdl-9256088
In many tissues, estrogen-induced vasodilatation is mediated, at least in part, by the release of nitric oxide (NO). We determined whether human myometrial endothelial and smooth muscle cells express estrogen receptors (ERs) and whether endothelial NO synthase (eNOS) expression in these cells was affected by 17beta-estradiol (10[-13]-10[-6]M). ER was strongly expressed in myometrial smooth muscle cells but was absent from endothelial cells. Expression of eNOS mRNA was strong in endothelial cells, but weak in muscle cells. 17beta-estradiol administration for 24 or 72 h failed to increase eNOS in both cell types. Thus, an increase of human uterine blood flow by estrogens appears not to be mediated by stimulation of myometrial eNOS expression.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Útero / Calcio / Óxido Nítrico Sintasa / Estradiol / Miometrio Límite: Adult / Aged / Female / Humans / Middle aged Idioma: En Revista: J Vasc Res Asunto de la revista: ANGIOLOGIA Año: 1997 Tipo del documento: Article País de afiliación: Austria Pais de publicación: Suiza
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Útero / Calcio / Óxido Nítrico Sintasa / Estradiol / Miometrio Límite: Adult / Aged / Female / Humans / Middle aged Idioma: En Revista: J Vasc Res Asunto de la revista: ANGIOLOGIA Año: 1997 Tipo del documento: Article País de afiliación: Austria Pais de publicación: Suiza