Characterisation of neprilysin (EC 3.4.24.11) S2' subsite.
FEBS Lett
; 411(1): 140-4, 1997 Jul 07.
Article
en En
| MEDLINE
| ID: mdl-9247159
Neprilysin is a neutral peptidase that cleaves small peptide substrates on the amino-side of hydrophobic amino acid residues. In the present study, we have used inhibition of non-mutated and mutated enzymes with dipeptide inhibitors and hydrolysis of the substrate [Leu5, Arg6]enkephalin in order to evaluate the contribution of the S2' subsite to substrate and inhibitor binding. Our results suggest that (1) Arg-102 and Asn-542 provide major contributions to the interaction of the enzyme with the P2' residue of the substrate, (2) the S2' subsite is vast and can accommodate bulky side chains, and (3) Arg-102 restricts access to the S2' subsite to some side chains such as arginine.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neprilisina
Límite:
Animals
Idioma:
En
Revista:
FEBS Lett
Año:
1997
Tipo del documento:
Article
País de afiliación:
Canadá
Pais de publicación:
Reino Unido