Protein/amphipathic polyamine complexes enable highly efficient transfection with minimal toxicity.

Budker, V; Hagstrom, J E; Lapina, O; Eifrig, D; Fritz, J; Wolff, J A

Budker, V; Hagstrom, J E; Lapina, O; Eifrig, D; Fritz, J; Wolff, J A.

Afiliación

Budker V; University of Wisconsin, Madison, USA.

Biotechniques ; 23(1): 139, 142-7, 1997 Jul.

Article en En | MEDLINE | ID: mdl-9232246

RESUMEN

Ternary complexes of plasmid DNA, histone H1 protein and amphipathic polyamines (PAPA) were able to mediate the efficient transfection of 3T3. HeLa and COS cells in culture. Using both the beta-galactosidase and luciferase reporter gene systems, the transfection efficiency of PAPA complexes was comparable to that of DOSPA/PE cationic liposomes, considered to be a highly-efficient transfection reagent. Using three different assays of cellular toxicity (propidium iodide, BCECF-AM and Trypan Blue), the PAPA complexes caused minimal cellular toxicity. These results indicate that PAPA complexes are useful transfection reagents for the study of gene expression and function in cultured cells.

Asunto(s)

Histonas/farmacología; Indicadores y Reactivos/farmacología; Poliaminas/farmacología; Transfección; Células 3T3; Animales; Células COS; Supervivencia Celular/efectos de los fármacos; Genes Reporteros; Células HeLa; Humanos; Ratones

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Poliaminas / Histonas / Transfección / Indicadores y Reactivos Límite: Animals / Humans Idioma: En Revista: Biotechniques Año: 1997 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido

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