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Pharmacology and mode of action of bradykinin on mouse-isolated trachea.
van Heuven-Nolsen, D; Westra-De Vlieger, J F; Muis, T; Denee, J H; Rivas, T O; Nijkamp, F P.
Afiliación
  • van Heuven-Nolsen D; Department of Pharmacology and Pathophysiology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, The Netherlands.
Naunyn Schmiedebergs Arch Pharmacol ; 356(1): 134-8, 1997 Jul.
Article en En | MEDLINE | ID: mdl-9228200
In the present study, the effect of bradykinin on basal and precontracted mouse-isolated trachea was investigated. In basal conditions mouse-isolated tracheal rings do not respond to bradykinin. However, when the tracheal rings were precontracted with carbachol (10(-7) M) a relaxation with bradykinin (3 x 10(-9)-3 x 10(-7)) was found. The maximal response amounted 69.7+/-4.1% (n=15) with a pD2 value of 7.2+/-0.21. The selective bradykinin B2 receptor antagonist HOE 140 (10(-10)-10(-8) M) antagonized the bradykinin-induced relaxation, while the bradykinin B1 receptor antagonist des-Arg9-Leu8-bradykinin (10(-6) M) had no influence. The selective bradykinin B1 receptor agonist des-Arg9-bradykinin (10(-6) M) caused a small relaxation (8.4+/-2.5%, n=6), which could be antagonized completely by the selective bradykinin B1 receptor antagonist des-Arg9-Leu8-bradykinin (10(-6) M) while addition of the selective bradykinin B2 receptor antagonist HOE 140 (10(-8) M) was without effect. In the presence of indomethacin (10(-6) M) the relaxation of bradykinin was completely abolished. Pretreatment of the tracheal rings with capsaicin, or the presence of the selective NK1 receptor antagonist RP 67851 (10(-6) M) or the presence of the nitric oxide synthase inhibitor L-NAME (3 x 10(-4) M) had no effect on the bradykinin-induced relaxation. In conclusion, these results demonstrate that the mouse-isolated tracheal is a preparation in which bradykinin exerts a relaxant response via stimulation of bradykinin B2 receptors. This response is probably mediated by prostaglandins.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tráquea / Bradiquinina / Contracción Muscular Límite: Animals Idioma: En Revista: Naunyn Schmiedebergs Arch Pharmacol Año: 1997 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Alemania
Buscar en Google
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Imprimir
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PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tráquea / Bradiquinina / Contracción Muscular Límite: Animals Idioma: En Revista: Naunyn Schmiedebergs Arch Pharmacol Año: 1997 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Alemania