Glucose-dependent transcriptional regulation of the human sucrase-isomaltase (SI) gene.

Rodolosse, A; Carrière, V; Chantret, I; Lacasa, M; Zweibaum, A; Rousset, M

Rodolosse, A; Carrière, V; Chantret, I; Lacasa, M; Zweibaum, A; Rousset, M.

Afiliación

Rodolosse A; Unité de Recherches sur la Différenciation Cellulaire Intestinale, INSERM U178, Villejuif, France.

Biochimie ; 79(2-3): 119-23, 1997.

Article en En | MEDLINE | ID: mdl-9209707

RESUMEN

We have previously shown that the transcription of the human sucrase-isomaltase (SI) gene was negatively regulated by glucose. Using two clonal metabolic variants of the human colon adenocarcinoma cell line Caco-2 we demonstrate here that: 1) although similar growth-related variations of phosphoenolpyruvate carboxykinase (PEPCK), frutose 1,6-diphosphatase (F1, 6-dPase), pyruvate kinase (PK) and SI mRNA levels are observed, only F1,6-dPase, PK and SI mRNA levels vary in the same way in response to modifications of glucose utilization; and 2) regulatory elements responsible for the glucose-dependent transcription of the SI gene are located within the -370/+30 region of the promoter.

Asunto(s)

Complejo Sacarasa-Isomaltasa/genética; Diferenciación Celular; Regulación Enzimológica de la Expresión Génica; Glucosa/metabolismo; Humanos; Regiones Promotoras Genéticas; ARN Mensajero/genética; Transcripción Genética; Células Tumorales Cultivadas

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Complejo Sacarasa-Isomaltasa Límite: Humans Idioma: En Revista: Biochimie Año: 1997 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Francia

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