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Oligotyping for HLA-DQA, -DQB, and -DPB in idiopathic nephrotic syndrome.
Haeffner, A; Abbal, M; Mytilineos, J; Konrad, M; Krammer, I; Bouissou, F; Opelz, G; Schärer, K; Cambon-Thomsen, A.
Afiliación
  • Haeffner A; Division of Pediatric Nephrology, University of Heidelberg, Germany.
Pediatr Nephrol ; 11(3): 291-5, 1997 Jun.
Article en En | MEDLINE | ID: mdl-9203175
Associations of human leukocyte antigens (HLA) with the idiopathic nephrotic syndrome (NS) have mainly been described for alleles of the HLA-DR locus. In the present study the polymorphism of HLA-DQ and -DP at the molecular level was investigated in 167 children with NS (129 steroid-sensitive) using the polymerase chain reaction and sequence-specific oligonucleotides in a French and a German cohort. HLA-DR typing was also performed by classical serology. In steroid-sensitive patients we observed an increased frequency of the alleles HLA-DQA1*0201 and -DQB1*0201 in both populations with relative risks ranging from 3.8 to 8.5 (Pb < 0.01 to Pb < 0.00001 after Bonferoni's correction). In contrast, the frequency of HLA-DQA1*0102 and DQB1*0602 was significantly decreased. In children with frequent relapses the HLA associations were generally more pronounced than in those with infrequent or no relapses. Applying logistic regression analysis, a nephrotic child bearing DQA1*0201 or DR7 was five times more likely to be in the steroid-sensitive group of patients than in the steroid-resistant group compared with nephrotic children not bearing one of these alleles. These HLA alleles therefore seem to be useful indicators of a steroid-sensitive frequently relapsing course of NS. No associations with DPB alleles were observed, which narrows the region genetically involved in the disease susceptibility to the DR-DQ region. Steroid-resistant NS was not associated with HLA.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Antígenos HLA-DP / Antígenos HLA-DQ / Síndrome Nefrótico Tipo de estudio: Diagnostic_studies / Etiology_studies / Risk_factors_studies Límite: Adolescent / Female / Humans / Male País/Región como asunto: Europa Idioma: En Revista: Pediatr Nephrol Asunto de la revista: NEFROLOGIA / PEDIATRIA Año: 1997 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Alemania
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Antígenos HLA-DP / Antígenos HLA-DQ / Síndrome Nefrótico Tipo de estudio: Diagnostic_studies / Etiology_studies / Risk_factors_studies Límite: Adolescent / Female / Humans / Male País/Región como asunto: Europa Idioma: En Revista: Pediatr Nephrol Asunto de la revista: NEFROLOGIA / PEDIATRIA Año: 1997 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Alemania