Survival of lactic acid bacteria in a dynamic model of the stomach and small intestine: validation and the effects of bile.
J Dairy Sci
; 80(6): 1031-7, 1997 Jun.
Article
en En
| MEDLINE
| ID: mdl-9201571
This study was conducted to validate a dynamic model of the stomach and small intestine to quantify the survival of lactic acid bacteria and to assess the influence of gastrointestinal secretions. The survival of a single strain of each of the following species, Bifidobacterium bifidum, Lactobacillus acidophilus, Lactobacillus bulgaricus, and Streptococcus thermophilus, was measured under physiological conditions (e.g., peristalsis, changes in pH, and changes in concentrations of enzymes and bile) and were compared with data obtained from humans. No significant differences were found between the in vitro and in vivo data, indicating that the model has a predictive value for the survival of these bacteria in humans. The survival of these strains of lactic acid bacteria in the gastrointestinal model was investigated under two different conditions in the small intestine: simulation of physiological secretion of bile and low bile secretion. Reductions in viability were significantly different between the bacterial species. The dose-response effect of bile on the survival of the tested bacteria was significant, demonstrating the bactericidal effect of bile salts. This study demonstrates the differences among bacterial species in their sensitivity to gastric and intestinal secretions.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Estómago
/
Streptococcus
/
Bifidobacterium
/
Ácidos y Sales Biliares
/
Intestino Delgado
/
Lactobacillus
/
Modelos Biológicos
Tipo de estudio:
Prognostic_studies
Límite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
J Dairy Sci
Año:
1997
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Estados Unidos