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Modulation of cytotoxicity and enhancement of cytokine release induced by Viscum album L. extracts or mistletoe lectins.
Ribéreau-Gayon, G; Jung, M L; Frantz, M; Anton, R.
Afiliación
  • Ribéreau-Gayon G; Laboratoire de Pharmacognosie, Université Louis Pasteur de Strasbourg, Illkirch, France.
Anticancer Drugs ; 8 Suppl 1: S3-8, 1997 Apr.
Article en En | MEDLINE | ID: mdl-9179359
Cytotoxic effects of Viscum album L. (mistletoe) extracts and mistletoe lectins were studied by light and electron microscopy. The first events observed were membrane perforation and protrusions typical for apoptosis. Inhibition of Molt 4 cell growth was obtained with lectin concentrations in the pg/ml range as long as cells were cultured in serum-free medium. Under this condition, mistletoe lectin-III was about 10 times more cytotoxic than mistletoe lectin-I; mistletoe lectin-II was in between. Lectin cytotoxicity was modulated by human serum from donors who had never been treated with mistletoe preparations and lectin-specific carbohydrates, added at the mmol/l range, particularly D-galactose (or beta-lactose) for mistletoe lectin-I and N-acetyl-galactosamine for mistletoe lectin-II and -III. In addition, at subtoxic concentrations, mistletoe lectin-I, -II and -III enhanced the production of cytokines (tumour necrosis factor-alpha, interleukin-1 alpha) by isolated human monocytes. The experimental results are discussed in relation to the treatment of cancer patients administered with mistletoe extracts.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Plantas Medicinales / Toxinas Biológicas / Muérdago / Citocinas / Preparaciones de Plantas / Lectinas / Antineoplásicos Fitogénicos Límite: Humans Idioma: En Revista: Anticancer Drugs Asunto de la revista: ANTINEOPLASICOS Año: 1997 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Plantas Medicinales / Toxinas Biológicas / Muérdago / Citocinas / Preparaciones de Plantas / Lectinas / Antineoplásicos Fitogénicos Límite: Humans Idioma: En Revista: Anticancer Drugs Asunto de la revista: ANTINEOPLASICOS Año: 1997 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido