Antibiotic resistance in Streptomyces lividans: fluorescence assay for streptogramin B lyase.

Bateman, K P; Armstrong, S M; White, R L; Ross, N W

Bateman, K P; Armstrong, S M; White, R L; Ross, N W.

Afiliación

Bateman KP; Department of Chemistry, Dalhousie University, Halifax, Nova Scotia, Canada.

Anal Biochem ; 248(2): 297-9, 1997 Jun 01.

Article en En | MEDLINE | ID: mdl-9177757

RESUMEN

A fluorescence assay for streptogramin B lyase, an enzyme that confers resistance to streptogramin B antibiotics, has been developed. The antibiotic substrates are fluorescent and the linear peptide products formed in the lyase-catalyzed reaction are relatively nonfluorescent. The assay has potential for assessing bacterial resistance to streptogramin B antibiotics and will be utilized to direct the purification of streptogramin B lyase from bacterial extracts.

Asunto(s)

Antibacterianos/farmacología; Liasas Intramoleculares; Isomerasas/análisis; Espectrometría de Fluorescencia/métodos; Streptomyces/efectos de los fármacos; Streptomyces/enzimología; Virginiamicina/farmacología; Farmacorresistencia Microbiana; Macrólidos; Especificidad por Sustrato

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría de Fluorescencia / Streptomyces / Virginiamicina / Liasas Intramoleculares / Isomerasas / Antibacterianos Idioma: En Revista: Anal Biochem Año: 1997 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos

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