Ultrastructural effects of lactoferrin binding on Giardia lamblia trophozoites.

Turchany, J M; McCaffery, J M; Aley, S B; Gillin, F D

Turchany, J M; McCaffery, J M; Aley, S B; Gillin, F D.

Afiliación

Turchany JM; Department of Pathology, University of California at San Diego 92103-8416, USA.

J Eukaryot Microbiol ; 44(1): 68-72, 1997.

Article en En | MEDLINE | ID: mdl-9172835

RESUMEN

Lactoferrin and its derived N-terminal peptide may be important host defenses against Giardia lamblia. We showed earlier that lactoferrin and the derived peptides have potent giardicidal activity in vitro. Using indirect immunofluorescence, we now demonstrate binding of lactoferrin and the peptides to the live trophozoite surface. Iron strongly inhibited binding of lactoferrin, and decreased binding of the peptides, while certain divalent metal ions decreased binding of all forms by about half. Lactoferrin and the peptides caused striking and complex morphologic changes in the trophozoite plasmalemma, endomembranes and cytoskeleton, and increased the electron density of the lysosome-like peripheral vacuoles.

Asunto(s)

Giardia lamblia/metabolismo; Lactoferrina/metabolismo; Animales; Giardia lamblia/ultraestructura; Fragmentos de Péptidos/metabolismo

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Giardia lamblia / Lactoferrina Límite: Animals Idioma: En Revista: J Eukaryot Microbiol Asunto de la revista: MICROBIOLOGIA / PARASITOLOGIA Año: 1997 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

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