Activity of H(+)-ATPase in ruminal bacteria with special reference to acid tolerance.
Appl Environ Microbiol
; 63(6): 2155-8, 1997 Jun.
Article
en En
| MEDLINE
| ID: mdl-9172333
Batch culture experiments showed that permeabilized cells and membranes of Ruminococcus albus and Fibrobacter succinogenes, acid-intolerant celluloytic bacteria, have only one-fourth to one-fifth as much H(+)-ATPase as Megasphaera elsdenii and Streptococcus bovis, which are relatively acid tolerant. Even in the cells grown in continuous culture at pH 7.0, the acid-intolerant bacteria contained less than half as much H(+)-ATPase as the acid-tolerant bacteria. The amounts of H(+)-ATPase in the acid-tolerant bacteria were increased by more than twofold when the cells were grown at the lowest pH permitting growth, whereas little increase was observed in the case of the acid-intolerant bacteria. These results indicate that the acid-intolerant bacteria not only contain smaller amounts of H(+)-ATPase at neutral pH but also have a lower capacity to enhance the level of H(+)-ATPase in response to low pH than the acid-tolerant bacteria. In addition, the H(+)-ATPases of the acid-intolerant bacteria were more sensitive to low pH than those of the acid-tolerant bacteria, although the optimal pHs were similar.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Rumen
/
Bacterias
/
ATPasas de Translocación de Protón
Límite:
Animals
Idioma:
En
Revista:
Appl Environ Microbiol
Año:
1997
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Estados Unidos