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Effects of an 11-day spaceflight on the choroid plexus of developing rats.
Mani-Ponset, L; Masseguin, C; Davet, J; Herbuté, S; Maurel, D; Ghandour, M S; Reiss-Bubenheim, D; Güell, A; Gabrion, J.
Afiliación
  • Mani-Ponset L; Dynamique Moleculaire des Interactions Membranaires, URA CNRS 1856, Universite de Montpellier II, Sciences et Techniques du Languedoc, France.
Brain Res Dev Brain Res ; 99(2): 187-200, 1997 Apr 18.
Article en En | MEDLINE | ID: mdl-9125472
Cellular distributions of ezrin, a cytoskeletal protein involved in apical cell differentiation in choroid plexus, and carbonic anhydrase II, which is partly involved in the cerebrospinal fluid production, were studied by immunocytochemistry, at the level of choroidal epithelial cells from the lateral, third and fourth ventricles in normal or experimental fetuses, in parallel with the ultrastructure of apical microvilli, observed by transmission electron microscopy. We compared choroid plexuses from developing normal rats (gestational day 15 to birth) with choroid plexuses from 20-day-old rat fetuses, developed for 11 days in space, aboard a space shuttle (NASA STS-66 mission, NIH-R1 experiments), from gestational day 9 to day 20. The main changes observed in fetuses developed in space were demonstrated by immunocytochemistry and concerned the distribution of ezrin and carbonic anhydrase II. Thus, in fetuses developing in space, ezrin was strongly detected in the choroidal cytoplasm and weakly associated to the membrane in the apical domain of the choroid plexus from the fourth ventricle. Such alterations suggested that choroid plexus from rat fetal brain displays a delayed maturation under a micro-gravitational environment. In contrast, intense immunoreactions to anti-carbonic anhydrase II antibodies showed that this enzyme is very abundant in rats developed in space, compared to ground control fetuses.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vuelo Espacial / Plexo Coroideo Límite: Animals / Pregnancy Idioma: En Revista: Brain Res Dev Brain Res Asunto de la revista: CEREBRO / NEUROLOGIA Año: 1997 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Países Bajos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vuelo Espacial / Plexo Coroideo Límite: Animals / Pregnancy Idioma: En Revista: Brain Res Dev Brain Res Asunto de la revista: CEREBRO / NEUROLOGIA Año: 1997 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Países Bajos