Steroid hormones act directly to trigger segment-specific programmed cell death of identified motoneurons in vitro.
Dev Biol
; 183(1): 95-107, 1997 Mar 01.
Article
en En
| MEDLINE
| ID: mdl-9119119
In larvae of the hawkmoth, Manduca sexta, accessory planta retractor (APR) motoneurons undergo a segment-specific pattern of programmed cell death at pupation. APR death is triggered hormonally by the prepupal peak of the ecdysteroid, 20-hydroxyecdysone (20-HE). Previous studies found no evidence that cellular interactions regulate the segmental pattern of APR death in vivo. To test the hypothesis that 20-HE acts directly on APRs to trigger a cell-autonomous, segment-specific pattern of death, APRs were labeled with the fluorescent dyes DiI or DiA, removed from the nervous system before the prepupal peak, and placed in low-density cell culture. Physiological levels of 20-HE triggered the same segment-specific pattern of APR death in vitro as seen in vivo, both in cultures containing a single APR and in cultures containing two APRs removed from the same donor animal. The presence or absence of contact with other cells did not influence the APRs' responses to 20-HE. The death of APRs in culture was characterized by fragmentation or rounding up of the cell body and fragmentation of the neurites. These findings suggest that intrinsic segmental identity regulates whether these motoneurons live or die when exposed to a steroid hormone during development.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Apoptosis
/
Manduca
/
Ecdisterona
/
Neuronas Motoras
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Dev Biol
Año:
1997
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos