Isolation and characterization of X chromosome-derived DNA sequences from a dioecious plant Melandrium album.

Buzek, J; Koutníková, H; Houben, A; Ríha, K; Janousek, B; Siroký, J; Grant, S; Vyskot, B

Buzek, J; Koutníková, H; Houben, A; Ríha, K; Janousek, B; Siroký, J; Grant, S; Vyskot, B.

Afiliación

Buzek J; Institute of Genetics and Molecular and Cellular Biology, Strasbourg, France.

Chromosome Res ; 5(1): 57-65, 1997 Feb.

Article en En | MEDLINE | ID: mdl-9088644

RESUMEN

A number of X chromosome DNA sequences have been isolated from a dioecious plant, Melandrium album (syn. Silene latifolia), using chromosome microdissection followed by degenerate oligonucleotideprimed polymerase chain reaction (DOP-PCR) amplification. Six DNA clones were selected and further characterized by DNA/DNA hybridization techniques to check their copy numbers, sex-specific methylation patterns, species specificity and positions on chromosomes. These clones were moderately to highly repetitive (approximately 10(3)-10(5) copies per haploid genome) and none of them gave a positive signal on Northern blots. One of the clones yielded a sex-specific methylation pattern: its abundant non-methylated CCGG island was found only in males. All the clones also hybridized to two closely related dioecious Melandrium species (M. rubrum and M. dicline). Nucleotide sequences of two X-derived clones showed a number of internal short direct repeats; one of them strikingly resembled a plant conservative telomere sequence (TTTAGGG). None of the clones hybridized to the X chromosome only, but all were localized at the telomeric heterochromatic regions (DAPI C-bands) of both arms of a vast majority of M. album chromosomes using the fluorescence in situ hybridization (FISH) technique. However, the non-homologous arm of the Y chromosome (contrary to the arm homologous to the X chromosome, possessing the pseudoautosomal region) showed neither a DAPI C-banding-stained heterochromatin nor a FISH signal with any of the DNA probes tested, thus indicating its evolutionary diversification.

Asunto(s)

Metilación de ADN; ADN de Plantas; Plantas/genética; Cromosoma X/genética; Composición de Base; Secuencia de Bases; Northern Blotting; Southern Blotting; Bandeo Cromosómico; Clonación Molecular; Sondas de ADN; ADN de Plantas/genética; ADN de Plantas/aislamiento & purificación; Heterocromatina/ultraestructura; Hibridación Fluorescente in Situ; Meiosis/genética; Mitosis/genética; Datos de Secuencia Molecular; Reacción en Cadena de la Polimerasa; Polimorfismo Genético; ARN de Planta/genética; ARN de Planta/aislamiento & purificación; Secuencias Repetitivas de Ácidos Nucleicos; Análisis de Secuencia de ADN; Cromatina Sexual; Especificidad de la Especie; Cromosoma X/ultraestructura; Cromosoma Y/genética; Cromosoma Y/ultraestructura

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plantas / Cromosoma X / ADN de Plantas / Metilación de ADN Idioma: En Revista: Chromosome Res Asunto de la revista: BIOLOGIA MOLECULAR Año: 1997 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Países Bajos

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links