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Endothelin-1 levels in portal venous blood in relation to hepatic tissue microcirculation disturbance and hepatic cell injury after ischemia/reperfusion.
Ota, T; Hirai, R; Urakami, A; Soga, H; Nawa, S; Shimizu, N.
Afiliación
  • Ota T; Second Department of Surgery, Okayama University Medical School, Japan.
Surg Today ; 27(4): 313-20, 1997.
Article en En | MEDLINE | ID: mdl-9086547
This study was conducted to clarify the role of endothelin-1 in the portal vein after hepatic ischemia/ reperfusion and to ascertain whether it is related to hepatic microcirculation disturbance. Using a canine ischemic liver model, the portal and systemic endothelin-1 levels were measured before ischemia, then after 1 h and 2 h of reperfusion, and comparatively evaluated with the serum levels of GOT and lactic dehydrogenase (LDH). As an indicator of liver tissue microcirculation, tissue blood flow volume (TBF) was also measured in the site subjected to ischemia. The animals were divided into: group 1, which received ischemia for 30 min; group 2, which received ischemia for 60 min; and group 3, which received a sequence repeated four times of 15 min ischemia and 10 min reperfusion. The portal endothelin-1 level became significantly elevated after reperfusion compared to that before ischemia in all groups, being significantly higher in group 2 than in the other groups. The systemic endothelin-1 level also increased after reperfusion; significantly in group 2. The portal endothelin-1 level was generally higher than the systemic level, which again was statistically significant in group 2. After 2 h of reperfusion, a significant positive correlation was found between the portal endothelin-I level and serum LDH, whereas a significant negative correlation was found between the portal endothelin-1 level and TBF. The finding that the portal endothelin-1 level became elevated after hepatic ischemia/reperfusion suggests that it probably plays an essential role in hepatic ischemia/ reperfusion injury by adversely influencing tissue microcirculation.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Daño por Reperfusión / Endotelina-1 / Circulación Hepática Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Surg Today Año: 1997 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Daño por Reperfusión / Endotelina-1 / Circulación Hepática Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Surg Today Año: 1997 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón