Evaluation of the transgenic Lambda/LacI mouse model as a short-term predictor of heritable risk.
Mutat Res
; 388(2-3): 129-36, 1997 Feb 14.
Article
en En
| MEDLINE
| ID: mdl-9057873
Transgenic male C57BL/6 lambda/lacI mice were used to assess the mutagenic response in seminiferous tubules and epididymal spermatozoa 3 days after exposure to ethylnitrosourea (ENU), iso-propyl methanesulfonate (iPMS) and methyl methanesulfonate (MMS). No significant mutagenic response was observed in epididymal spermatozoa for all three compounds, as expected 3 days after treatment. However, ENU and iPMS treated samples demonstrated significant mutagenic inductions relative to controls in seminiferous tubules while MMS treated samples did not. The failure of MMS to induce a mutagenic response in lambda/lacI transgenic mice is likely due to a combination of the low dose used, the short expression time after exposure and the reduced sensitivity to large deletion events in transgenic lambda/lacI shuttle vectors. In addition, ex vivo mutations were measured in control samples and iPMS treated samples, where 33% of mutants from control samples and 35% of mutants from iPMS treated samples were mosaic.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Mutación de Línea Germinal
/
Mesilatos
/
Proteínas de Escherichia coli
/
Etilnitrosourea
/
Metilmetanosulfonato
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Pruebas de Mutagenicidad
Tipo de estudio:
Diagnostic_studies
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Etiology_studies
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Evaluation_studies
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Prognostic_studies
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Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
Mutat Res
Año:
1997
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Países Bajos