Nitrogen mustard-mediated mutagenesis in human T-lymphocytes in vitro.

Olsen, L S; Korsholm, B; Nexø, B A; Wassermann, K

Olsen, L S; Korsholm, B; Nexø, B A; Wassermann, K.

Afiliación

Olsen LS; Department of Toxicology and Biology, National Institutes of Occupational Health, Copenhagen, Denmark.

Arch Toxicol ; 71(3): 198-201, 1997.

Article en En | MEDLINE | ID: mdl-9049058

RESUMEN

The cytotoxic and mutagenic effect of the bifunctional alkylating agent nitrogen mustard (HN2) was examined. Primary human lymphocytes were exposed to graded doses of HN2 in vitro and relative survival was determined. Mutation induction at the hypoxanthine-guanine phosphoribosyltransferase (hprt) locus was measured by cloning the exposed T-cells in microtitre plates in the presence and absence of 6-thioguanine (TG). The IC50-value determined for 30 min exposure to HN2 was 1.34 microM. The mutant frequencies (MF) in exposed T-cell cultures were 10-fold (2 microM HN2) to 32-fold (4 microM HN2) higher than those of unexposed cultures (median values). Nitrogen mustard-mediated mutagenesis is discussed in terms of the current ideas about DNA damage and repair.

Asunto(s)

Sustancias para la Guerra Química/toxicidad; Mecloretamina/toxicidad; Mutágenos/toxicidad; Linfocitos T/efectos de los fármacos; Supervivencia Celular/efectos de los fármacos; Células Clonales; Humanos; Técnicas In Vitro; Masculino

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos T / Sustancias para la Guerra Química / Mecloretamina / Mutágenos Límite: Humans / Male Idioma: En Revista: Arch Toxicol Año: 1997 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Alemania

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