Activation of a cyclic nucleotide phosphodiesterase 4 (PDE4) from rat thymocytes by phosphatidic acid.
Cell Signal
; 8(7): 511-6, 1996 Nov.
Article
en En
| MEDLINE
| ID: mdl-9023016
The cytosolic cyclic nucleotide phosphodiesterase (PDE) activity from rat thymocytes was resolved into five peaks by HPLC. Only two forms of the cAMP-specific PDE4 were found to be sensitive to physiologically relevant phosphatidic acid (PA) concentrations. PA activated the PDE4-peak 3 form, the fatty acid composition and unsaturation degree determining the efficiency of PA. The PDE4-peak 2 form was inhibited only by PA with saturated fatty acyl groups. PDE4 activation was specific of anionic phospholipids, a free phosphate group in the phospholipid molecule being required for maximum activation. These results suggest that PA may contribute to the lowering of cAMP level required in the early steps of a lympho-proliferative response, thus regulating immune functions through PDE4 activation.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ácidos Fosfatidicos
/
Timo
/
3',5'-AMP Cíclico Fosfodiesterasas
/
Hidrolasas Diéster Fosfóricas
Límite:
Animals
Idioma:
En
Revista:
Cell Signal
Año:
1996
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Reino Unido