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Structure and expression of a cluster of glutathione S-transferase genes from a marine fish, the plaice (Pleuronectes platessa).
Leaver, M J; Wright, J; George, S G.
Afiliación
  • Leaver MJ; NERC Unit of Aquatic Biochemistry, University of Stirling, Scotland, U.K.
Biochem J ; 321 ( Pt 2): 405-12, 1997 Jan 15.
Article en En | MEDLINE | ID: mdl-9020873
Glutathione S-transferases are involved in the detoxification of reactive electrophilic compounds, including intracellular metabolites, drugs, pollutants and pesticides. A cluster of three glutathione S-transferase genes, designated GSTA, GSTA1 and GSTA2, was isolated from the marine flatfish, plaice (Pleuronectes platessa). GSTA and GSTA1 code for protein products with 76% amino acid identity. GSTA2 appears to contain a single nucleotide deletion which would render any product non-functional. All of these genes consist of six exons of similar sizes and greater than 70% nucleotide identity, and are interrupted by five introns of differing sizes. GSTA and GSTA1 mRNAs were present in a range of tissues, while GSTA2 mRNA was no detected. Expression of GSTA mRNA was increased in plaice intestine and spleen by pretreatment with beta-naphthoflavone, and expression of both GSTA and GSTA1 mRNAs was increased in plaice liver and gill by pretreatment with the peroxisome proliferating agent perfluoro-octanoic acid.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Peces Planos / Familia de Multigenes / Genes / Glutatión Transferasa Límite: Animals Idioma: En Revista: Biochem J Año: 1997 Tipo del documento: Article Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Peces Planos / Familia de Multigenes / Genes / Glutatión Transferasa Límite: Animals Idioma: En Revista: Biochem J Año: 1997 Tipo del documento: Article Pais de publicación: Reino Unido