The UL2 open reading frame of bovine herpesvirus 1 encodes a uracil-DNA glycosylase.
Microbiol Immunol
; 40(12): 949-53, 1996.
Article
en En
| MEDLINE
| ID: mdl-9013493
Sequence analysis within the unique long segment of the bovine herpesvirus 1 (BHV-1) genome previously identified an open reading frame (ORF), designated UL2, whose deduced polypeptide of 204 amino acids contained a consensus uracil-DNA glycosylase (UDGase) signature sequence. To determine whether the BHV-1 UL2 ORF product has UDGase activity, we positioned the UL2 sequence down-stream of the T7 promoter on the vector pET-28b(+) and expressed it in Escherichia coli. Upon induction with isopropyl beta-D-thiogalactopyranoside these cells produced a 23-kDa protein, the molecular mass of which was in accordance with the prediction from the nucleotide sequence. A one-step purification procedure using nickel-chelating affinity chromatography resulted in a homogeneous preparation of this protein, which displayed specific UDGase activity in an in vitro enzyme assay. These results provide evidence that the BHV-1 UL2 gene does encode a UDGase.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Herpesvirus Bovino 1
/
ADN Glicosilasas
/
Genes Virales
/
N-Glicosil Hidrolasas
Idioma:
En
Revista:
Microbiol Immunol
Año:
1996
Tipo del documento:
Article
País de afiliación:
Taiwán
Pais de publicación:
Australia