[Serologic and skin test diagnosis of birch pollen allergy with recombinant Bet v 1, the chief allergen of birch]. / Serologische und Hauttest-Diagnostik der Birkenpollenallergie mit rekombinantem Bet v 1, dem Hauptallergen der Birke.
Pneumologie
; 50(9): 632-40, 1996 Sep.
Article
en De
| MEDLINE
| ID: mdl-9011868
BACKGROUND: Type I allergy represents a severe health problem in industrialized countries where up to 20% of the population suffers from allergic rhinitis, conjunctivitis and allergic asthma bronchiale and in severe cases from anaphylaxis, leading to death. OBJECTIVE: The aim of this study was to evaluate recombinant Bet v 1, the major birch pollen allergen for in vivo and in vitro diagnosis of birch pollen allergy. METHODS: A group of 51 birch pollen allergic patients and eight non-allergic control individuals were tested for birch pollen allergy by skin-prick and intradermal testing, comparing commercial birch pollen extracts with recombinant Bet v 1. Quantitative and qualitative serological testing was done with natural and recombinant allergens by radioallergosorbent test (RAST), enzyme-linked immunosorbent assay (ELISA) and immunoblotting. RESULTS: Recombinant Bet v 1 allowed accurate in vivo and in vitro diagnosis of tree pollen allergy in 49/51 patients tested. No false positive results were obtained in any in vitro assay system (ELISA, Westernblot) or by skin testing (skin-prick, intradermal test) with recombinant Bet v 1. CONCLUSION: Our results document that recombinant Bet v 1 produced in bacterial expression systems allows accurate in vitro and in vivo diagnosis of birch pollen allergy in > 95% of birch pollen allergic patients.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas de Plantas
/
Polen
/
Hipersensibilidad Respiratoria
/
Asma
/
Pruebas Intradérmicas
/
Alérgenos
/
Hipersensibilidad Inmediata
Tipo de estudio:
Diagnostic_studies
/
Qualitative_research
Límite:
Adult
/
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
De
Revista:
Pneumologie
Año:
1996
Tipo del documento:
Article
Pais de publicación:
Alemania