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A Drosophila gene regulated by rough and glass shows similarity to ena and VASP.
DeMille, M M; Kimmel, B E; Rubin, G M.
Afiliación
  • DeMille MM; Howard Hughes Medical Institute, Department of Molecular and Cell Biology University of California, Berkeley 94720, USA.
Gene ; 183(1-2): 103-8, 1996 Dec 12.
Article en En | MEDLINE | ID: mdl-8996093
rough (ro) encodes a homeobox transcription factor required for proper specification of photoreceptor cells R2 and R5 in Drosophila eye development. To identify the transcriptional targets through which ro acts to specify the R2/R5 neuronal sub-type, we screened enhancer trap lines expressed in developing photoreceptors for those whose expression patterns were altered when ro function was inactivated. In this way we identified two potential ro targets, which are also targets of the zinc finger transcription factor glass (gl). We also identified an enhancer trap line that exhibits altered morphogenetic furrow expression in a ro mutant background. Finally, we have molecularly characterized an enhancer trap line, AE33, that was identified in earlier screens as a target of both ro and gl (freeman et al., 1992; Treisman and Rubin, 1996). The transcript interrupted by AE33 shares similarity with the mammalian vasodilator-stimulated phosphoprotein (VASP), a substrate for cAMP- and cGMP-dependent protein kinases that is associated with actin filaments, focal adhesions, and dynamic membrane regions (Haffner et al., 1995) with enabled (ena), a substrate of the Drosophila Abl tyrosine kinase (Gertler et al. 1995) and with two human Expressed Sequence Tags (ESTs).
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Células Fotorreceptoras de Invertebrados / Regulación del Desarrollo de la Expresión Génica / Proteínas de Ciclo Celular / Proteínas de Drosophila / Proteínas de Unión al ADN / Drosophila melanogaster / Proteínas Asociadas a Microtúbulos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Gene Año: 1996 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Células Fotorreceptoras de Invertebrados / Regulación del Desarrollo de la Expresión Génica / Proteínas de Ciclo Celular / Proteínas de Drosophila / Proteínas de Unión al ADN / Drosophila melanogaster / Proteínas Asociadas a Microtúbulos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Gene Año: 1996 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos