Recombinant Hansenula polymorpha as a biocatalyst: coexpression of the spinach glycolate oxidase (GO) and the S. cerevisiae catalase T (CTT1) gene.
Appl Microbiol Biotechnol
; 46(1): 46-54, 1996 Aug.
Article
en En
| MEDLINE
| ID: mdl-8987534
The methylotrophic yeast Hansenula polymorpha has been developed as an efficient production system for heterologous proteins. The system offers the possibility to cointegrate heterologous genes in anticipated fixed copy numbers into the chromosome. As a consequence co-production of different proteins in stoichiometric ratios can be envisaged. This provides options to design this yeast as an industrial biocatalyst in procedures where several enzymes are required for the efficient conversion of a given inexpensive compound into a valuable product. To this end recombinant strains have been engineered with multiple copies of expression cassettes containing the glycolate oxidase (GO) gene from spinach and the catalase T (CTT1) gene from S. cerevisiae. The newly created strains produce high levels of the peroxisomal glycolate oxidase and the cytosolic catalase T. The strains efficiently convert glycolate into glyoxylic acid, oxidizing the added substrate and decomposing the peroxide formed during this reaction into water and oxygen.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Pichia
/
Proteínas Recombinantes
/
Catalasa
/
Expresión Génica
/
Oxidorreductasas de Alcohol
Idioma:
En
Revista:
Appl Microbiol Biotechnol
Año:
1996
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Alemania