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Systemic T cell-independent tumor immunity after transplantation of universal receptor-modified bone marrow into SCID mice.
Hege, K M; Cooke, K S; Finer, M H; Zsebo, K M; Roberts, M R.
Afiliación
  • Hege KM; Department of Immunology and Cell Biology, Cell Genesys Inc., Foster City, California 94404, USA.
J Exp Med ; 184(6): 2261-9, 1996 Dec 01.
Article en En | MEDLINE | ID: mdl-8976181
Gene modification of hematopoietic stem cells (HSC) with antigen-specific, chimeric, or "universal" immune receptors (URs) is a novel but untested form of targeted immunotherapy. A human immunodeficiency virus (HIV) envelope-specific UR consisting of the extracellular domain of human CD4 linked to the zeta chain of the T cell receptor (CD4 zeta) was introduced ex vivo into murine HSC by retroviral transduction. After transplantation into immunodeficient SCID mice, sustained high level expression of CD4 zeta was observed in circulating myeloid and natural killer cells. CD4 zeta-transplanted mice were protected from challenge with a lethal dose of a disseminated human leukemia expressing HIV envelope. These results demonstrate the ability of chimeric receptors bearing zeta-signaling domains to activate non-T cell effector populations in vivo and thereby mediate systemic immunity.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptores de Antígenos de Linfocitos T / Linfocitos T / Antígenos CD4 / Productos del Gen env / Trasplante de Médula Ósea / VIH / Proteínas de la Membrana Límite: Animals / Female / Humans / Male Idioma: En Revista: J Exp Med Año: 1996 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptores de Antígenos de Linfocitos T / Linfocitos T / Antígenos CD4 / Productos del Gen env / Trasplante de Médula Ósea / VIH / Proteínas de la Membrana Límite: Animals / Female / Humans / Male Idioma: En Revista: J Exp Med Año: 1996 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos