Immunoglobulin light chain staining in paraffin-embedded tissue using a heat mediated epitope retrieval method.
Histopathology
; 29(6): 525-31, 1996 Dec.
Article
en En
| MEDLINE
| ID: mdl-8971559
We have compared light chain immunohistochemistry in reactive lymphoid tissue and a series of paraffin-embedded B-cell lymphomas using standard trypsin digestion with a heat mediated epitope retrieval method. Fifty-seven B-cell lymphomas (18 high grade, 29 low grade and 10 cases of nodular lymphocyte predominance Hodgkin's disease), two reactive lymph nodes and eight tonsils fixed for known times between 12 h and 2 years were studied. Paraffin-embedded tissue was stained with polyclonal anti-kappa and anti-lambda antibodies. For each antibody staining was performed on two sections, one treated with trypsin digestion and one with microwave heating. Sections were scored from 0 to + + + with 0 representing poor staining and + + + excellent staining. A score of ++ was considered satisfactory. Light chain restriction was recorded if present. Satisfactory staining was obtained in 34/59 cases using trypsin digestion and 56/59 cases using heat retrieval. Light chain restriction was demonstrated in 32/57 (56%) B-cell lymphomas using trypsin digestion and 52/57 (91%) using heat retrieval. Satisfactory staining was obtained in tonsils fixed for up to 48 h using trypsin digestion and up to 2 years using heat retrieval. We have shown that for light chain immunostaining a heat mediated epitope retrieval method produces more consistent and satisfactory results and is effective over a greater range of fixation times than traditional trypsin digestion.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Inmunohistoquímica
/
Linfoma de Células B
/
Cadenas Ligeras de Inmunoglobulina
/
Tejido Linfoide
Límite:
Humans
Idioma:
En
Revista:
Histopathology
Año:
1996
Tipo del documento:
Article
Pais de publicación:
Reino Unido