Evaluation of two commercial microtiter cytotoxin assays for the detection of Clostridium difficile toxin B in stool specimens.

Fedorko, D P; Williams, E C

Fedorko, D P; Williams, E C.

Afiliación

Fedorko DP; Clinical Pathology Department, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892, USA.

Diagn Microbiol Infect Dis ; 26(1): 47-51, 1996 Sep.

Article en En | MEDLINE | ID: mdl-8950530

RESUMEN

Two commercial microtiter cytotoxin assays using a fibroblast cell line (Bartels, Baxter Diagnostics, Inc., Deerfield, IL) and an epithelial cell line (Cytotoxi Test, Advanced Clinical Diagnostics, Toledo, OH) were evaluated for their ability to detect Clostridium difficile toxin B in stool specimens. After 48 hours, the assays had comparable sensitivity (90 versus 92%) and specificity (99 versus 98%). Although not statistically significant, the Bartels assay detected more toxin-positive specimens at 24 hours (84 versus 72%, P = 0.089, Fisher's Exact Test) and the Cytotoxi assay had fewer nonspecific reactions requiring repeat testing (2.2 versus 1.1%, P = 0.186, Fisher's Exact Test). Both cytotoxin assays had comparable analytic performance.

Asunto(s)

Bioensayo/métodos; Clostridioides difficile/química; Citotoxinas/aislamiento & purificación; Enterocolitis Seudomembranosa/diagnóstico; Células Cultivadas; Reacciones Falso Negativas; Reacciones Falso Positivas; Heces/microbiología; Fibroblastos; Humanos; Sensibilidad y Especificidad

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bioensayo / Enterocolitis Seudomembranosa / Clostridioides difficile / Citotoxinas Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Diagn Microbiol Infect Dis Año: 1996 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

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