Cloning and characterization of the gene encoding the OmpU outer membrane protein of Vibrio cholerae.

Sperandio, V; Bailey, C; Girón, J A; DiRita, V J; Silveira, W D; Vettore, A L; Kaper, J B

Sperandio, V; Bailey, C; Girón, J A; DiRita, V J; Silveira, W D; Vettore, A L; Kaper, J B.

Afiliación

Sperandio V; Center for Vaccine Development, University of Maryland School of Medicine, Baltimore 21201, USA.

Infect Immun ; 64(12): 5406-9, 1996 Dec.

Article en En | MEDLINE | ID: mdl-8945596

RESUMEN

The OmpU outer membrane protein is a member of the ToxR regulon of Vibrio cholerae and has recently been shown to be a potential adherence factor for this species. Using PCR and degenerate oligonucleotide primers based on internal peptide sequences of purified OmpU, we have cloned and sequenced the gene encoding OmpU. The ompU gene is predicted to encode a 36,646-molecular-weight protein which is present in both cholera toxin-positive and -negative V. cholerae O1 and O139 strains.

Asunto(s)

Adhesinas Bacterianas/genética; Proteínas de la Membrana Bacteriana Externa/genética; Vibrio cholerae/genética; Secuencia de Aminoácidos; Secuencia de Bases; Clonación Molecular; Datos de Secuencia Molecular

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de la Membrana Bacteriana Externa / Vibrio cholerae / Adhesinas Bacterianas Tipo de estudio: Prognostic_studies Idioma: En Revista: Infect Immun Año: 1996 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

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