A Brevibacterium linens pRBL1 replicon functional in Corynebacterium glutamicum.

Ankri, S; Bouvier, I; Reyes, O; Predali, F; Leblon, G

Ankri, S; Bouvier, I; Reyes, O; Predali, F; Leblon, G.

Afiliación

Ankri S; Institut de Génétique et Microbiologie URA, Université de Paris-Sud, Orsay, France.

Plasmid ; 36(1): 36-41, 1996 Jul.

Article en En | MEDLINE | ID: mdl-8938050

RESUMEN

Brevibacterium linens RBL strain cryptic plasmid pRBL1 (8.0 kb) is described. A region involved in pRBL1 autonomous replication in Corynebacterium glutamicum was identified by insertion and deletion mapping and partially sequenced. This region encodes for a hypothetical 310-amino acid (aa) protein closely related to the replicases of plasmids pXZ10142 (C. glutamicum) and pAL5000 (Mycobacterium fortuitum). The 310-aa protein also shows significant homology to proteins of pColE5-099 (Shigella sonnei) and pJD1 (Neisseria gonorrhoea). At least one of these proteins, the Rep protein of pColE5-099, is known to be involved in theta replication.

Asunto(s)

Brevibacterium/genética; Corynebacterium/genética; Vectores Genéticos/fisiología; Plásmidos/fisiología; Replicón/fisiología; Secuencia de Aminoácidos; Proteínas Bacterianas/genética; Proteínas Bacterianas/aislamiento & purificación; Clonación Molecular; Datos de Secuencia Molecular

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plásmidos / Replicón / Brevibacterium / Corynebacterium / Vectores Genéticos Idioma: En Revista: Plasmid Año: 1996 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos

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