ALS IgGs suppress [Ca2+]i rise through P/Q-type calcium channels in central neurones in culture.

Andjus, P R; Khiroug, L; Nistri, A; Cherubini, E

Andjus, P R; Khiroug, L; Nistri, A; Cherubini, E.

Afiliación

Andjus PR; International School for Advanced Studies (SISSA), Trieste, Italy.

Neuroreport ; 7(12): 1914-6, 1996 Aug 12.

Article en En | MEDLINE | ID: mdl-8905691

RESUMEN

Confocal laser scanning microscopy (with the fluorescent calcium dye fluo-3) was used to test the effect of IgG obtained from patients with amyotrophic lateral sclerosis (ALS) on the KCl-induced [Ca2+] rise in rat hippocampal neurones in culture. In the presence of tetrodotoxin and ionotropic glutamate receptor antagonists, ALS IgGs depressed (by 30-40%) Ca2+ transients evoked by influx of Ca2+ through voltage-activated channels; such an effect did not occur with IgG obtained from healthy donors. The depressant action of ALS IgG was selectively prevented by the inhibitor of P/Q-type Ca2+ channels, omega-agatoxin IVA (which alone reduced Ca2+ transients by 40%). The reduced Ca2+ transients might impair Ca(2+)-dependent glutamate receptor desensitization and thus facilitate excitotoxic damage.

Asunto(s)

Esclerosis Amiotrófica Lateral/metabolismo; Canales de Calcio/efectos de los fármacos; Calcio/metabolismo; Hipocampo/efectos de los fármacos; Inmunoglobulina G/farmacología; Animales; Canales de Calcio/metabolismo; Células Cultivadas; Hipocampo/metabolismo; Ratas

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inmunoglobulina G / Canales de Calcio / Calcio / Hipocampo / Esclerosis Amiotrófica Lateral Límite: Animals Idioma: En Revista: Neuroreport Asunto de la revista: NEUROLOGIA Año: 1996 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido

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