Competitive ELISA for detection of HHV-6 antibody: seroprevalence in a danish population.
J Virol Methods
; 56(2): 221-30, 1996 Feb.
Article
en En
| MEDLINE
| ID: mdl-8882652
A competitive HHV-6 enzyme-linked immunosorbent assay (ELISA) was established, utilizing HHV-6 rabbit polyclonal antibodies and HHV-6 cellular antigen. In total, 285 sera from Danish children aged 1-6 years were screened for HHV-6 antibodies. The lowest seropositive rate, 78% was found in the youngest age group from 1 year to 15 months. All 120 (100%) children aged 3 years and above were seropositive. The ELISA was compared with indirect immunofluorescence assay (IFA) using a group of 83 serum samples from 65 Danish patients (aged 4 weeks to 72 years). All but one of the sera (100% of patients) were positive in the ELISA, but only 71% of sera (65% of patients) were positive with both assays. The ELISA detected one seroconversion in a baby, which was not detected by IFA. The ELISA was found to be more sensitive than IFA.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ensayo de Inmunoadsorción Enzimática
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Herpesvirus Humano 6
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Infecciones por Herpesviridae
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Anticuerpos Antivirales
Tipo de estudio:
Diagnostic_studies
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Prevalence_studies
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Risk_factors_studies
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Screening_studies
Límite:
Adolescent
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Adult
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Aged
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Animals
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Child
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Child, preschool
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Humans
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Infant
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Male
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Middle aged
País/Región como asunto:
Europa
Idioma:
En
Revista:
J Virol Methods
Año:
1996
Tipo del documento:
Article
Pais de publicación:
Países Bajos