Primary structure of bovine adenosine deaminase.

Kelly, M A; Vestling, M M; Murphy, C M; Hua, S; Sumpter, T; Fenselau, C

Kelly, M A; Vestling, M M; Murphy, C M; Hua, S; Sumpter, T; Fenselau, C.

Afiliación

Kelly MA; Department of Chemistry and Biochemistry, University of Maryland Baltimore County 21228, USA.

J Pharm Biomed Anal ; 14(11): 1513-9, 1996 Aug.

Article en En | MEDLINE | ID: mdl-8877857

RESUMEN

Derivatized bovine adenosine deaminase is used in enzyme replacement therapy and as an adjunct to gene therapy against severe combined immunodeficiency syndrome. Although a gene sequence is known for human adenosine deaminase, the structure of the bovine enzyme has not been characterized. Structure studies using mass spectrometry are reported here that evaluate sequence, processing, post-translational modifications and the extent of homology between the human protein and its therapeutic surrogate.

Asunto(s)

Adenosina Desaminasa/química; Adenosina Desaminasa/genética; Secuencia de Aminoácidos; Animales; Bovinos; Humanos; Espectrometría de Masas; Datos de Secuencia Molecular; Homología de Secuencia de Aminoácido

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Adenosina Desaminasa Límite: Animals / Humans Idioma: En Revista: J Pharm Biomed Anal Año: 1996 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido

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