Field and pore size dependence of the electrophoretic mobility of DNA: a combination of fluorescence recovery after photobleaching and electric birefringence measurements.
Electrophoresis
; 17(6): 1046-51, 1996 Jun.
Article
en En
| MEDLINE
| ID: mdl-8832170
By combining an electrophoretic cell with a setup of fluorescence recovery after photobleaching (FRAP) we can measure the electrophoretic mobility mu of double-stranded lambda DNA in agarose gel as a function of electric field E and gel concentration C. Mobility varies linearly with the field in agreement with the biased reptation model with fluctuations. The slopes are analyzed in term of orientation and compared with birefringence results. The mobility extrapolated at zero field follows the prediction of the reptation theory; we deduced the variation of the pore size with the agarose concentration. With a special use of our setup, we measure directly the free-mobility mu 0 of the DNA.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Compuestos de Quinolinio
/
Benzoxazoles
/
ADN Viral
/
Electroforesis en Gel de Agar
/
Colorantes Fluorescentes
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Electrophoresis
Año:
1996
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Alemania