Transcription of the AAC1 gene encoding an isoform of mitochondrial ADP/ATP carrier in Saccharomyces cerevisiae is regulated by oxygen in a heme-independent manner.
Eur J Biochem
; 239(3): 759-63, 1996 Aug 01.
Article
en En
| MEDLINE
| ID: mdl-8774724
The mitochondrial ADP/ATP carrier in Saccharomyces cerevisiae is encoded by three independent genes. AAC1, AAC2, and AAC3. In this work, we analysed the 5' upstream region of AAC1 by sequencing and by mapping the transcription initiation site of the gene. By monitoring the level of AAC1 mRNA and the beta-galactosidase activity of AAC1-lacZ fusion constructs, we showed that expression of AAC1 is subjected to regulation by oxygen. In contrast to the other two AAC genes, the effect of oxygen on AAC1 is not mediated by heme and heme-dependent transcription factors. The AAC1 expression was reduced eightfold in anaerobically grown cells compared to expression in cells grown aerobically, but it was not affected by the nature of carbon source used for growth. The data presented show that AAC1 expression, while constitutive under all aerobic conditions tested, is repressed during anaerobiosis in a heme-independent manner.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oxígeno
/
Saccharomyces cerevisiae
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Transcripción Genética
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Translocasas Mitocondriales de ADP y ATP
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Regulación Fúngica de la Expresión Génica
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Mitocondrias
Idioma:
En
Revista:
Eur J Biochem
Año:
1996
Tipo del documento:
Article
País de afiliación:
Eslovaquia
Pais de publicación:
Reino Unido