A simple assay to determine the functionality of Cre or FLP recombination targets in genomic manipulation constructs.
Nucleic Acids Res
; 24(15): 3118-9, 1996 Aug 01.
Article
en En
| MEDLINE
| ID: mdl-8760904
We report the construction of two Escherichia coli strains (294-Cre and 294-FLP) which express either Cre- or FLP-recombinase. Plasmids containing authentic recognition targets for either recombinase (loxPs or FRTs) are recombined when propagated in the appropriate strain. 294-Cre and 294-FLP thus provide a simple test for the recombination competence of constructs that are designed for use in Cre- or FLP-mediated genomic manipulations.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Recombinación Genética
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Proteínas Virales
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Ingeniería Genética
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Integrasas
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ADN Nucleotidiltransferasas
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Escherichia coli
Idioma:
En
Revista:
Nucleic Acids Res
Año:
1996
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Reino Unido