Resection and mutagenesis of the acid pH-inducible P2 promoter of the Agrobacterium tumefaciens virG gene.
J Bacteriol
; 178(15): 4717-20, 1996 Aug.
Article
en En
| MEDLINE
| ID: mdl-8755905
Transcription of the virG gene initiates from two tandem promoters, designated P1 and P2, that are located 50 nucleotides apart. Transcription of the P2 promoter is induced by extracellular acidity. cis-acting sites required for P2 activity were identified by constructing and assaying a series of 5' and 3' resections and site-directed nucleotide substitutions. Nucleotides between positions -9 and -37 were sufficient for regulated promoter activity. Within this region, nucleotide substitutions at the predicted -10 and -35 regions strongly reduced P2 expression. In addition, alterations in the region between nucleotides -24 and -32 also eliminated or strongly reduced promoter activity. These data suggest that this promoter may be regulated by a positive transcription factor that binds to nucleotide residues in this interval.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Factores de Transcripción
/
Regiones Promotoras Genéticas
/
Agrobacterium tumefaciens
/
Proteínas de Unión al ADN
/
Genes Bacterianos
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
J Bacteriol
Año:
1996
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos