3-dimensional morphometry of intact dendritic spines observed in thick sections using an electron microscope.
J Neurosci Methods
; 62(1-2): 73-82, 1995 Nov.
Article
en En
| MEDLINE
| ID: mdl-8750087
An experimental technique is described which allows observation of fixed neuronal dendrites at magnifications from 10-12 K. The method uses 4-7-microns-thick sections of Epon-embedded tissue with nerve cells that are first impregnated by the rapid Golgi technique and then stained with gold particles/aggregates using a modified gold-toning procedure. A relatively high acceleration voltage (200 kV) is employed to observe in fine detail the dendritic fragments of interest at different angular positions in space, by using a eucentric goniometer stage with a tilt angle of +/- 45 degrees. Image analysis methodology is proposed which permits estimation of 3-dimensional (3D) lengths and of the volume of observed intact dendritic spines. The advantages of the technique with respect to 3D reconstruction methodology are discussed.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Microscopía Electrónica
/
Dendritas
Límite:
Animals
Idioma:
En
Revista:
J Neurosci Methods
Año:
1995
Tipo del documento:
Article
Pais de publicación:
Países Bajos