Determination of phenylbutazone and oxyphenbutazone in plasma and urine samples of horses by high-performance liquid chromatography and gas chromatography-mass spectrometry.
J Chromatogr B Biomed Appl
; 678(2): 211-8, 1996 Apr 12.
Article
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| MEDLINE
| ID: mdl-8738024
A method is described for the qualitative and quantitative determination of phenylbutazone and oxyphenbutazone in horse urine and plasma samples viewing antidoping control. A horse was administered intravenously with 3 g of phenylbutazone. For the qualitative determination, a screening by HPLC was performed after acidic extraction of the urine samples and the confirmation process was realized by GC-MS. Using the proposed method it was possible to detect phenylbutazone and oxyphenbutazone in urine for up to 48 and 120 h, respectively. For the quantitation of these drugs the plasma was deproteinized with acetonitrile and 20 microliters were injected directly into the HPLC system equipped with a UV detector and LiChrospher RP-18 column. The mobile phase used was 0.01 M acetic acid in methanol (45:55, v/v). The limit of detection was 0.5 microgram/ml for phenylbutazone and oxyphenbutazone and the limit of quantitation was 1.0 microgram/ml for both drugs. Using the proposed method it was possible to quantify phenylbutazone up to 30 h and oxyphenbutazone up to 39 h after administration.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oxifenilbutazona
/
Fenilbutazona
/
Cromatografía Líquida de Alta Presión
/
Caballos
/
Cromatografía de Gases y Espectrometría de Masas
Tipo de estudio:
Qualitative_research
Límite:
Animals
Idioma:
En
Revista:
J Chromatogr B Biomed Appl
Año:
1996
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Países Bajos