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Quantitative and qualitative alterations of dystrophin are expressed in muscle cell cultures of Xp21 muscular dystrophy patients (Duchenne and Becker type).
Mongini, T; Doriguzzi, C; Palmucci, L; Chiadò-Piat, L.
Afiliación
  • Mongini T; Paolo Peirolo Centre for Neuromuscular Diseases, University of Turin, Italy.
Eur J Clin Invest ; 26(4): 322-4, 1996 Apr.
Article en En | MEDLINE | ID: mdl-8732491
The authors studied skeletal muscle cell cultures from four control subjects, two patients with Duchenne muscular dystrophy, two Duchenne carriers and three patients with Becker muscular dystrophy with different phenotypes. Western blotting was performed on well-differentiated myotubes and compared with the results obtained in muscle tissue. In all cultures the band of dystrophin closely corresponded to the one observed in muscle tissue: both quantitative and qualitative defects were observed. This confirms the early expression of the Xp21 gene defect in uninnervated muscle cultures and supports the usefulness of muscle cultures both in diagnostic procedure and as a model to study the disease.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromosoma X / Distrofina / Distrofias Musculares Tipo de estudio: Prognostic_studies / Qualitative_research Límite: Adult / Child / Humans / Male Idioma: En Revista: Eur J Clin Invest Año: 1996 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromosoma X / Distrofina / Distrofias Musculares Tipo de estudio: Prognostic_studies / Qualitative_research Límite: Adult / Child / Humans / Male Idioma: En Revista: Eur J Clin Invest Año: 1996 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido