Occurrence of deoxyribonucleic acid fragmentation during prolactin-induced structural luteolysis in cycling rats.
Biol Reprod
; 54(6): 1245-51, 1996 Jun.
Article
en En
| MEDLINE
| ID: mdl-8724351
We determined whether fragmentation of genomic DNA, one of the hallmarks of apoptosis, occurs during structural luteolysis in cycling rats. Corpora lutea (CL) were collected from rats at each estrous cycle stage (1800 h), and fragmented DNA was extracted. Only CL from rats at the proestrous stage showed distinct DNA fragmentation. To determine the period of occurrence of DNA fragmentation, CL were collected at several points between 1200 h on the day of proestrus and 0600 h on the day of estrus. Distinct DNA fragmentation was observed from 1800 h (proestrus) to 2400 h (proestrus), and the extent was significantly lower at 0600 h (estrus). It is known that prolactin (PRL) induces structural luteolysis in rats. To examine the role of PRL in luteal DNA fragmentation, 2-bromo-alpha-ergocryptine (BE) was used to suppress the PRL surge on the day of proestrus. CL collected at 1800 h from BE-treated rats did not show distinct DNA fragmentation, and PRL injection offset the effect of BE. Histochemical analysis with a 3'-end labeling technique confirmed the occurrence of DNA fragmentation in luteal tissue. These results suggest that apoptotic cell death occurs during PRL-induced structural luteolysis.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Prolactina
/
Daño del ADN
/
Estro
Límite:
Animals
Idioma:
En
Revista:
Biol Reprod
Año:
1996
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Estados Unidos