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Glutathione depletion induces glycogenolysis dependent ascorbate synthesis in isolated murine hepatocytes.
Braun, L; Csala, M; Poussu, A; Garzó, T; Mandl, J; Bánhegyi, G.
Afiliación
  • Braun L; Department of Medical Chemistry, Semmelweis University of Medicine, Budapest, Hungary.
FEBS Lett ; 388(2-3): 173-6, 1996 Jun 17.
Article en En | MEDLINE | ID: mdl-8690080
The relationship between glutathione deficiency, glycogen metabolism and ascorbate synthesis was investigated in isolated murine hepatocytes. Glutathione deficiency caused by various agents increased ascorbate synthesis with a stimulation of glycogen breakdown. Increased ascorbate synthesis from UDP-glucose or gulonolactone could not be further affected by glutathione depletion. Fructose prevented the stimulated glycogenolysis and ascorbate synthesis caused by glutathione consumption. Reduction of oxidised glutathione by dithiothreitol decreased the elevated glycogenolysis and ascorbate synthesis in diamide or menadione treated hepatocytes. Our results suggest that a change in GSH/GSSG ratio seems to be a sufficient precondition of altering glycogenolysis and a consequent ascorbate synthesis.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácido Ascórbico / Glutatión / Glucógeno / Hígado Límite: Animals Idioma: En Revista: FEBS Lett Año: 1996 Tipo del documento: Article País de afiliación: Hungria Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácido Ascórbico / Glutatión / Glucógeno / Hígado Límite: Animals Idioma: En Revista: FEBS Lett Año: 1996 Tipo del documento: Article País de afiliación: Hungria Pais de publicación: Reino Unido