Cloning of a gene encoding a DNase I-like endonuclease in the human Xq28 region.
Gene
; 168(2): 267-70, 1996 Feb 12.
Article
en En
| MEDLINE
| ID: mdl-8654957
To contribute to the isolation of genes within the q-24-qter region of the human X chromosome,we screened three cDNA libraries (human fetal brain, liver and skeletal muscle) with a cosmid clone containing a CpG island previously mapped in the q28 region. A full-length 2.1-kb cDNA clone was isolated (XIB); DNA databank searches revealed identity with an EST fragment (XAP-1), residing between the RCP/GCP and G6PD loci. The XIB coding region (909 bp) showed 44% amino acid (aa) identity to pig DNase I. Several conserved residues have been observed between these two genes including aa in the active site. XIB expressed a single transcript in adult heart and skeletal muscle, whereas, in some fetal tissues, two different-sized transcripts were seen. Zoo blot analysis showed a remarkable cross-species conservation. Expression and sequence of this novel gene are reported.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cromosoma X
/
Desoxirribonucleasa I
Límite:
Adult
/
Animals
/
Humans
Idioma:
En
Revista:
Gene
Año:
1996
Tipo del documento:
Article
País de afiliación:
Italia
Pais de publicación:
Países Bajos