Possible nystatin-protein interaction in yeast plasma membrane vesicles in the presence of ergosterol. A Förster energy transfer study.

Opekarová, M; Urbanová, P; Konopásek, I; Kvasnicka, P; Strzalka, K; Sigler, K; Amler, E

Opekarová, M; Urbanová, P; Konopásek, I; Kvasnicka, P; Strzalka, K; Sigler, K; Amler, E.

Afiliación

Opekarová M; Insitute of Microbiology, Czech Academy of Sciences, Prague, Czech Republic. opekaro@biomed.cas.cz

FEBS Lett ; 386(2-3): 181-4, 1996 May 20.

Article en En | MEDLINE | ID: mdl-8647277

RESUMEN

The Förster energy transfer from tryptophan residues of membrane proteins to nystatin was measured in reconstituted yeast plasma membrane vesicles free of, or doped with, ergosterol. We wanted to elucidate whether the functional change of membrane transport proteins from H+ symporters to facilitators, observed in ergosterol-containing plasma membrane vesicles on addition of nystatin [Opekarová and Tanner (1994) FEBS Lett. 350, 46-50], is reflected in altered protein-nystatin relations within the membrane. Both frequency-domain and time-domain time-resolved fluorescence spectroscopy showed that in the presence of ergosterol nystatin is located much closer to membrane proteins than in its absence.

Asunto(s)

Ergosterol/farmacología; Proteínas de la Membrana/metabolismo; Nistatina/metabolismo; Saccharomyces cerevisiae/metabolismo; Membrana Celular/metabolismo; Transferencia de Energía; Cómputos Matemáticos; Modelos Moleculares; Unión Proteica/efectos de los fármacos; Saccharomyces cerevisiae/efectos de los fármacos; Triptófano

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Nistatina / Ergosterol / Proteínas de la Membrana Idioma: En Revista: FEBS Lett Año: 1996 Tipo del documento: Article País de afiliación: República Checa Pais de publicación: Reino Unido

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