Enhanced production of succinic acid by overexpression of phosphoenolpyruvate carboxylase in Escherichia coli.

Millard, C S; Chao, Y P; Liao, J C; Donnelly, M I

Millard, C S; Chao, Y P; Liao, J C; Donnelly, M I.

Afiliación

Millard CS; Department of Chemical Engineering, Texas A&M University, College Station 77843, USA.

Appl Environ Microbiol ; 62(5): 1808-10, 1996 May.

Article en En | MEDLINE | ID: mdl-8633880

RESUMEN

Fermentative production of succinic acid from glucose by Escherichia coli was significantly increased by overexpression of phosphoenolpyruvate carboxylase. In contrast, overexpression of phosphoenolpyruvate carboxykinase had no effect. Under optimized conditions, induction of the carboxylase resulted in a 3.5-fold increase in the concentration of succinic acid, making succinic acid the major fermentation product by weight.

Asunto(s)

Escherichia coli/metabolismo; Fosfoenolpiruvato Carboxilasa/biosíntesis; Succinatos/metabolismo; Escherichia coli/genética; Técnicas de Transferencia de Gen; Fosfoenolpiruvato Carboxilasa/genética; Ácido Succínico

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfoenolpiruvato Carboxilasa / Succinatos / Escherichia coli Idioma: En Revista: Appl Environ Microbiol Año: 1996 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

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