Eosinophil chemotactic activity in bronchoalveolar lavage fluid obtained from Toxocara canis-infected rats.
Clin Immunol Immunopathol
; 78(3): 256-62, 1996 Mar.
Article
en En
| MEDLINE
| ID: mdl-8605701
We examined eosinophil chemotactic activity (ECA) in bronchoalveolar lavage fluid (BALF) obtained from rats infected with Toxocara canis. For 4 weeks after infection, the number of eosinophils was determined in peripheral blood and BALF. ECA was assayed using a microchemotaxis chamber. Eosinophils in peripheral blood and BALF increased markedly after infection, peaking at 12 days and 2 weeks, respectively. ECA in BALF also increased significantly and peaked 2 weeks after infection. Partial characterization revealed that ECA was heat labile, lipid soluble, and resistant to trypsin digestion. Two ECA peaks were identified by molecular sieve column chromatography: one near the egg albumin marker (MW 45,000) and the other observed after elution with quinacrine (MW 472.9). Treatment with a specific leukotriene (LT) B4 receptor antagonist (ONO-4057), a platelet activating factor (PAF) receptor antagonist (TCV-309), and an anti-interleukin (IL)-5 monoclonal antibody (TB13) significantly reduced the ECA, suggesting that LTB4, PAF, and IL-5 contribute to the accumulation of eosinophils in the lungs of rats infected with T. canis.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Toxocariasis
/
Quimiotaxis de Leucocito
/
Toxocara canis
/
Receptores de Superficie Celular
/
Tetrahidroisoquinolinas
/
Receptores Acoplados a Proteínas G
/
Eosinófilos
/
Pulmón
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Clin Immunol Immunopathol
Año:
1996
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Estados Unidos