Interaction of an anti-HIV peptide, T22, with gp120 and CD4.
Biochem Biophys Res Commun
; 219(2): 555-9, 1996 Feb 15.
Article
en En
| MEDLINE
| ID: mdl-8605026
T22 ([Tyr5,12, Lys7]-polyphemusin II) has been shown to have strong anti-human immunodeficiency virus (HIV) activity. The precise mechanism of action of T22 on HIV-replication has not been elucidated yet, nor have the targets of T22 been identified. However, our previous research suggested that T22 exerts its effect by blocking virus-cell fusion and that T22 might interact with an HIV envelope protein and/or a T-cell surface protein. Herein we use a novel biosensor based on the principles of surface plasmon resonance (BIAcore) to demonstrate that T22 binds specifically to both gp120 (an envelope protein of HIV) and CD4 (a T-cell surface protein) and that both bindings can be inhibited by an anti-T22 antibody. The data obtained suggest that T22 inhibits virus-cell fusion through the double binding to the above two proteins.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Antivirales
/
Péptidos
/
Antígenos CD4
/
Proteína gp120 de Envoltorio del VIH
/
VIH-1
/
Péptidos Catiónicos Antimicrobianos
Límite:
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
1996
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Estados Unidos