Comparison of three methods for competitive binding of monoclonal antibodies. The localization of antigenic sites for monoclonal antibodies on Panulirus interruptus hemocyanin.
J Immunol Methods
; 190(1): 117-25, 1996 Mar 28.
Article
en En
| MEDLINE
| ID: mdl-8601704
The competitive binding of a panel of monoclonal antibodies against hemocyanin of Panulirus interruptus hemocyanin was investigated with three different methods. A competitive-binding immunoassay method was more successful in the determination of ternary complexes than gel electrophoresis and gel filtration. The latter two methods could only be applied with antibodies possessing a higher affinity. Eleven monoclonal antibodies were assigned to groups on the basis of their interactions with five antigenic regions.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Hemocianinas
/
Mapeo Epitopo
/
Crustáceos
/
Anticuerpos Monoclonales
Límite:
Animals
Idioma:
En
Revista:
J Immunol Methods
Año:
1996
Tipo del documento:
Article
País de afiliación:
Países Bajos
Pais de publicación:
Países Bajos