Association between alpha-hemolysin production and HeLa cell-detaching activity in fecal isolates of Escherichia coli.
J Clin Microbiol
; 33(10): 2707-9, 1995 Oct.
Article
en En
| MEDLINE
| ID: mdl-8567910
Escherichia coli isolates that cause detachment of cell monolayers during in vitro adherence assays (cell-detaching E. coli [CDEC]) were recently reported as a potential new group of enteropathogenic bacteria. In the present study, 269 E. coli isolates from feces of children 1 to 5 years of age were identified as CDEC in a detaching assay developed with HeLa cells. The great majority of these isolates were hemolytic within 3 h of growth on blood agar plates and hybridized with a DNA probe for alpha-hemolysin (93.7%), while most of the non-detaching isolates were hemolytic within 24 h (3.6%) or nonhemolytic (94.8%). E. coli isolates that produced alpha-hemolysin were found in 60 (30%) of 200 children with diarrhea and 47 (24%) of 200 age-matched controls. No statistical significance was found for the differences in alpha-hemolysin production among the matched pairs (P = 0.2). These data suggest that CDEC isolates are not associated with diarrhea in the population studied.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Adhesión Celular
/
Proteínas de Escherichia coli
/
Diarrea
/
Escherichia coli
/
Infecciones por Escherichia coli
/
Heces
/
Proteínas Hemolisinas
Tipo de estudio:
Risk_factors_studies
Límite:
Child, preschool
/
Humans
/
Infant
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
J Clin Microbiol
Año:
1995
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Estados Unidos