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Peripheral tolerance in T cell receptor-transgenic mice: evidence for T cell anergy.
Falb, D; Briner, T J; Sunshine, G H; Bourque, C R; Luqman, M; Gefter, M L; Kamradt, T.
Afiliación
  • Falb D; ImmuLogic Pharmaceutical Corporation, Waltham, USA.
Eur J Immunol ; 26(1): 130-5, 1996 Jan.
Article en En | MEDLINE | ID: mdl-8566055
T cell tolerance can be induced in adult mice by injection of soluble antigenic peptide. The underlying mechanism has been difficult to establish in normal mice due to the low precursor frequency of T cells specific for any given antigen. Therefore, we examined peripheral tolerance in mice transgenic for a T cell receptor specific for a cytochrome c peptide bound to I-Ek. Antigen-specific hyporesponsiveness could be induced in the transgenic mice. We followed the transgene-bearing T cells with a clonotypic monoclonal antibody and found similar numbers of clonotypic T cells in tolerized and control mice. To prevent de novo differentiation of T cells we analyzed thymectomized mice in which antigen-specific hyporesponsiveness was induced. Our analysis of thymectomized transgenic mice showed that antigen-specific T cell hyporesponsiveness following injection of peptide intravenously is not caused by gross elimination of T cells. These data provide evidence for the role of anergy in peripheral tolerance.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos T / Receptores de Antígenos de Linfocitos T alfa-beta / Anergia Clonal / Tolerancia Inmunológica Límite: Animals Idioma: En Revista: Eur J Immunol Año: 1996 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos T / Receptores de Antígenos de Linfocitos T alfa-beta / Anergia Clonal / Tolerancia Inmunológica Límite: Animals Idioma: En Revista: Eur J Immunol Año: 1996 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania