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Visceral leishmaniasis: use of the polymerase chain reaction in an epidemiological study in Baringo District, Kenya.
Schaefer, K U; Schoone, G J; Gachihi, G S; Muller, A S; Kager, P A; Meredith, S E.
Afiliación
  • Schaefer KU; Department of Infectious Diseases, University of Amsterdam, The Netherlands.
Trans R Soc Trop Med Hyg ; 89(5): 492-5, 1995.
Article en En | MEDLINE | ID: mdl-8560520
The polymerase chain reaction was applied to capillary blood spots dried on filter paper from 20 parasitologically proved cases of visceral leishmaniasis (VL), 21 subclinical cases, and 11 healthy controls in a longitudinal study of anthroponotic VL in Baringo District, Kenya. Leishmania deoxyribonucleic acid (DNA) was detected 10.5 months before diagnosis and up to 3 years after diagnosis and apparently successful treatment. Subclinical cases can have detectable circulating parasite DNA in their blood. These findings may indicate that subclinical cases can be a reservoir and formerly treated VL patients can remain a reservoir for a long time. Xenodiagnosis should be performed on subclinical cases and former VL patients to establish their role in transmission of VL in Kenya.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leishmaniasis Visceral Tipo de estudio: Observational_studies / Risk_factors_studies Límite: Adolescent / Adult / Animals / Child / Child, preschool / Female / Humans / Male País/Región como asunto: Africa Idioma: En Revista: Trans R Soc Trop Med Hyg Año: 1995 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leishmaniasis Visceral Tipo de estudio: Observational_studies / Risk_factors_studies Límite: Adolescent / Adult / Animals / Child / Child, preschool / Female / Humans / Male País/Región como asunto: Africa Idioma: En Revista: Trans R Soc Trop Med Hyg Año: 1995 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Reino Unido